Adaptive optics for microscopy

Practical guides to implementation

A repository for beginners guides, hints, tips and tricks on how to implement adaptive optics in microscopes and related optical systems. Hosted by the Dynamic Optics and Photonics Group, Department of Engineering Science, University of Oxford.

Adaptive Optics is a valuable tool that can enhance the performance of a broad range of optical systems, but it is often considered challenging to implement. This website provides a range of information to help understand and implement this technology, particularly for microscopy and related methods.

To learn more about the motivation for the site, see the About page. The documents themselves can be found in the Documents section below. If you don’t know where to start, the Basic introduction document gives a broad introduction to the field. Finally, to give feedback or ask any questions, please use the Contact form.

This experimental protocol describes several procedures related to the operation of an imaging system based on wavefront control and step-index multimode optical fibres (MMFs). It is assumed that such an imaging system is already built and aligned, without an MMF. This protocol aims at providing guidance to new users of the system.
This user manual explains how to install and use the svmPSF plugins for Fiji/ImageJ. The svmPSF plugin models a spatially variant PSF into a series of spatially invariant PSFs for image deconvolution.